Nanotechnologies. 5-(and 6)-Chloromethyl-2’,7’ Dichlorodihydrofluorescein diacetate (CM-H2DCF- DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line shop-only ISO/TR 19664 assists by providing

ISO/TR 19664 assists by providing clarity regarding the limitations that can be expected when using different instrumentation and equipment for the assessment of daily vibration exposure at the workplace

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Nanotechnologies. 5-(and 6)-Chloromethyl-2’,7’ Dichlorodihydrofluorescein diacetate (CM-H2DCF- DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line shop-only ISO/TR 19664 assists by providing1 Scope This document describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264. 7 macrophage cells exposed to nano objects, nanoparticles, their aggregates and agglomerates using the CM H2DCFDA assay. The protocol in this document is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.