CSM Single Drop out formulation-csm-single:CSM, Single Drop-Out -Iso: 740 mg/l

CSM Single Drop out formulation-csm-single:CSM, Single Drop-Out -Iso: 740 mg/lCSM is also a basis for many Drop Out mixtures to select for auxotrophic requirements and transformants. Each CSM Single Drop Out mixture contain all components except for one essential nutrient, i. e. the dropped out supplement. Some CSM Single Drop Out mixtures are also available with additions of extra Amino Acids and other components. Store all Drop out mixtures dry at room temperature

1% Heavy metals >10ppm Residue on ignition >0

Appearance White Crystalline Powder Identification (FTIR) Positive Solubility (100 mg/L) Clear and colourless solution Purity (HPLC) >92% Potency >1000 µg/mg Heavy metals <20 ppm Specific rotation 22

Friehs and Flaschel (2004)

30 H3BO3 1

55 gram powdered medium in 1 litre distilled water

The hydroxyl group present at the 3 position of G-418 is phosphorylated by NPTII

Synthetic Defined Minimal Media

5 Thin Layer Chromatogram One Spot Potency >720 µ/mg Store at 2 - 8°C

0 (10 g/l) Assay ≥99%

01 Folic Acid 0

glycerol is not fermented to acetic acid

Soluble in water (20°C