Terrific Broth Size:6 x 1kg

Terrific Broth Size:6 x 1kgTerrific Broth is rich nutritional medium developed by Tartoff and Hobbs supporting luxurious high density growth of E. coli and a higher yield of plasmid DNA compared to LB type media. Recombinant E. coli cells have an extended growth phase in Terrific medium. Terrific Broth medium is used with glycerol as a carbon source. Unlike glucose, glycerol is not fermented to acetic acid. Tryptone and Yeast are present in the medium in elevated concentrations

Chemical formula C2H5NO2 Solubility (20° C) 225 g/l Mol

Glucose 11000 g/l KH2PO4 680

9% Purity (TLC) One Spot IR Conform standard Solubility (5%

Karl Friehs and Erwin Flaschel

6 g/l) Assay ≥99%

Tryptone 8 Yeast extract 5 NaCl 5 Agar 15 Final pH 7

minus Cysteine

Suspend 37gram powdered medium in 1 litre distilled water

Tetracycline is a bacteriostatic antibioticum with activity against gram-positive and gram-negative bacteria

Concentrations of Cysteine

4°C Water KF 0

These Amino acids form a basis for many "Drop-Out" mixtures to select for auxotrophic requirements and transformants